This paper examines the impact of a multicultural approach on the usability of web and software interface designs. Through the use of an eye-tracking system, the study compares the ability of American users to navigate traditional American and Japanese websites. The ASL R6 eye-tracking system recorded user search latency and the visual scan path in locating specific items on the American and Japanese pages. Experimental results found statistically significant latency values when searching for left- or right-oriented navigation menus. Among the participants, visual observations of scan paths indicated a strong preference for initial movements toward the left. These results demonstrate the importance of manipulating web layouts and navigation menus for American and Japanese users. This paper further discusses the potential strengths resulting from modifications of interface designs to correspond with such cultural search tendencies, and suggestions for further research.